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Bio Rad QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications. BioRad QX200 combines water-oil emulsion droplet technology with microfluidics. The AutoDG droplet generator partitions each sample into 20,000 uniform nanoliter-sized droplets and a droplet reader bundled QuantaSoft™ software count the PCR-positive and PCR-negative droplets.
Thousands of hospitals and molecular testing labs in the US and around the world have Bio-Rad’s QX200 and QXDx ddPCR systems installed and have the capacity to perform SARS-CoV-2 ddPCR testing. The SARS-CoV-2 ddPCR test represents the latest addition to Bio-Rad’s product offerings related to COVID-19. This is the second EUA Bio-Rad has been granted for COVID-19. Last week, the company received an EUA for a serology test to detect antibodies to the virus.
Droplet Digital PCR (ddPCR) employs advanced microfluidics technology to generate thousands of highly uniform nanoliter-sized droplets in each sample, achieving sample partitioning on a massive scale. Once generated, the droplets can be amplified, read and analyzed. In traditional PCR, a single measurement is performed on a single sample, while in ddPCR, thousands of measurement can be performed on each sample.
Bio-Rad ddPCR can be useful for many food/water quality and safety applications such, GMO testing, allergens detection, food fraud, bacterial and foodborne/waterborne virus enumeration and virulence profiling. In this area of application the technology offers all its intrinsic benefits in terms of resolution in quantification possibility of genes colocalization at single bacterium level and reduced sensitivity to PCR inhibitors.
Benefits of the QX200 Droplet Digital PCR System
Most precise and sensitive digital PCR solution for a wide variety of applications
Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
Flexible assay setup — scalable for high sensitivity or high throughput
Simple and easy-to-use workflow with 96-sample throughput
Droplet partitioning by the QX200 Droplet Digital technology reduces bias from amplification efficiency and PCR inhibitors
Convenient assay design — standard curves are not required
Applications of the QX200 Droplet Digital PCR System
Cancer biomarker studies and copy number variation Measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution. PrimePCR™ ddPCR Assays are now available for mutation and copy number detection. These are predesigned, wet-lab validated assays.
Pathogen detection Employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring.
Next generation sequencing Perform accurate quantification and qualification of NGS libraries without the use of a standard curve.
Gene expression analysis Achieve reliable and reproducible measurements of small fold changes for low abundance of mRNA and miRNA.
Environmental monitoring Test a wide variety of environmental samples like soil and water using the QX200 System.
Food testing Perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods.
Include:
QX200 Droplet Generator system
QX200 Droplet Reader system
dedicated analysis notebook PC
1PX1 PCR Plate Sealer
