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Live Cell Imaging & High-Precision Fluorescence Microscopy High-Throughput Automated Imaging Phase Contrast & DIC Imaging Pathology & Clinical Diagnostics Immunofluorescence Studies Cell Biology & Microbiology Research Oncology & Cancer Research Neuroscience & Developmental Biology Pharmaceutical & Biotech Research
Trinocular
Zeiss Infinity-Corrected Optics
Explore the Zeiss Axio Observer Z1 for superior imaging in life sciences. Automated 3D sectioning & super-resolution with robust ZEISS optics for precise analysis
Description
The Zeiss Axio Observer Z1 is a highly versatile inverted microscope system designed to meet the dynamic requirements of life sciences research. It provides interfaces for various technologies, from widefield transmitted light to sensitive super-resolution imaging and convenient 3D sectioning, making it suitable for complex imaging tasks. Researchers can choose the optimal incubation equipment for their experiments and benefit from easy sample access, which is essential for precise micromanipulation.
This system is particularly beneficial for materials science applications such as metallography. The inverted design of the Axio Observer allows for efficient investigation, development, and analysis of materials, especially metallographic specimens. Combining ZEISS’s renowned optics quality with automated components ensures reliability and reproducibility in research findings.
Features of the Carl Zeiss Axio Observer Z1 Inverted Microscope include:
Optics for image generation
High thermal and mechanical stability
The high degree of flexibility for documenting results
Improved ergonomics
LCD for instrument parameters
Instrument parameters are displayed on and can be configured using a TFT display
23 mm field of view
Light Manager, Contrast Manager
Modular design for optimum adaptation for different applications
6-position nosepiece
6-position reflector turret
can be loaded in situ or removed for loading
5 or 6-position condenser turret
3-position Optovar turret
Removable aperture stop and luminous-field stop sliders for reflected light
Fluorescence shutter (internal standard shutter or external high-speed shutter)
HAL 100, HBO 100, HBO 50 illuminators
All essential microscope functions are motorized
Specifications:
Zeiss Axio Observer Z1 motorized
Opto-electronically encoded motorized XY stage 130*85 mm travel. Inserts for slides, 35 mm dishes and multiwell plates.
Pecon XL S1 incubator and control modules
X-CITE 120XL metal halide fluorescence light source
Filter cubes:
Analyzer
DAPI filter cube 49 (BP G365, FT 395, BP 445/50)
Cy5 filter cube (Semrock FF01-630/38, FF655-Di01, FF01-694/44)
GFP filter cube HE38 (BP 470/40, FT 495, BP 525/50)
YFP filter cube HE46 (BP 500/20, FT 515, BP 535/30)
Red filter cube HQ45 (BP 560/40, FT 585, BP 630/75)
FRET cube available (Semrock x438/24, FF458-Di01, m542/27) – swap into position 4 for ratiometric CFP/YFP FRET
Also available (please ask LMCF staff for them):
CFP filter cube HE47 (BP 436/20, FT 455, BP 480/40)
Cy7 filter cube (BP710/75, FT750, BP810/90)
Fluorescence attenuator:
Open (100% Transmission)
70% T
50% T
40% T
20% T
2% T
Objectives:
2.5x/0.075 EC-Plan-NeoFluar (440310-9903), WD: 9.5mm
5x/0.16 Plan-NeoFluar (440320-9902), WD: 18.5mm
10x/0.30 Plan-NeoFluar Ph1 (440331-9902), WD: 5.2mm
20x/0.80 Plan-Apochromat DICII (440640-9903), WD: 0.55mm, Coverglass: 0.17mm
63x/1.4 Oil Plan Apochromat DICIII (440762-9904), WD: 0.19mm, Coverglass: 0.17mm
100x/1.46 (Oil) Plan Apochromat DICIII (420792-9800), WD: 0.11mm, Coverglass: 0.17mm, (UV)VIS-IR
Available: 40x/1.3 Oil Plan-NeoFluar DICII (440452-9903), WD: 0.20mm, Coverglass: 0.17mm
Available: 40x/0.75 EC-Plan-NeoFluar DICII (440350-9903), WD: 0.71mm, Coverglass: 0.17mm
Calibrations for Photometrics Evolve EMCCD and CoolSNAP ES2 cameras:
2.5x EMCCD px=6.1650 um, CoolSNAP px=2.420 um
5x EMCCD px= 3.0828 um, CoolSNAP px= 1.264 um
10x EMCCD px= 1.5556 um, CoolSNAP px= 0.613 um
20x EMCCD px= 0.7726 um, CoolSNAP px= 0.309 um
40x EMCCD px= 0.3810 um, CoolSNAP px= 0.157 um
63x EMCCD px= 0.2423 um, CoolSNAP px= 0.098 um
100x EMCCD px= 0.1558 um, CoolSNAP px=0.062 um
Cameras:
Photometrics Evolve back-thinned EMCCD camera (512*512)
Photometrics CoolSNAP HQ2 CCD camera (1392*1040)
Computer:
HP Z4 G4 Workstation, Intel W-2133 3.6GHz processor, 32GB RAM, Nvidia Quadro P620 2GB graphics
Windows 10 64-bit
MetaMorph 7.10.1, system ID 32330
ZEN Pro 2.3
Include:
Zeiss Power Supply 232
Zeiss 425537 binocular tube 45⁰/23
Zeiss 455043-0000 W-Pl 10x/23 eyepiece set
Zeiss 6 Place Nosepiece, H DIC Motorized
Zeiss 420140-9900 Fluar 10x/0.50 ∞/0.17
Zeiss 421360-9970 LD Plan-Neofluar 40x/0.6 Korr ∞/0-1.5
Zeiss 421380-9970 LD Plan-Neofluar 63x/0.75 Korr ∞/0-1.5
Zeiss 424947 6 position fluorescence reflector turret motorized with four fluorescence cubes, DAPI, FITC, TR. CY3
Zeiss 424244 6 Place Motorized LD NA 0.55 WD 26mm turret condenser with Ph1, Ph2, and Ph3 annuli with open space to add DIC prisms.
Zeiss 1x C-Mount on the left side. The microscope has an additional camera port on the right side.
Zeiss HAL100 100W Halogen Lamp for transmitted light illumination.
Zeiss HBO 100 Lamp with Power Supply for fluorescence.
