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Xenon flash lamp (250 Hz) with 10-year warranty on new instruments
UV-Vis 150,000 nm/min
OpenLab Software Suite
Cary 3500 Multicell UV-Vis Spectrophotometer
The Cary 3500 Multicell UV-Vis is a double beam spectrophotometer offering new, unique measurement capabilities that can be deployed in regulated environments. The system simultaneously measures up to four independent temperature experiments across eight cuvette positions, with air-cooled Peltier temperature control. A xenon flash lamp—complete with 10-year replacement warranty (for Cary 3500 instruments purchased from Agilent or participating partners)—eliminates the daily warm-up time and drastically reduces the frequency and cost of lamp replacement. You can also accurately monitor kinetic experiments with a data collection rate up to 250 points per second.
The Cary 3500 comes complete with Cary UV Workstation software—a next-generation analytical platform with streamlined methods for wavelength reads, scanning, concentration, kinetics, and temperature measurements. When configured with Agilent OpenLab data management software, Cary UV Workstation data is managed, protected, and stored in a local or central secure database.
Measure samples at four temperatures across eight cuvette positions in a single experiment and collect data from each cuvette simultaneously.
Control the temperature of your samples accurately and quickly from -5 to 110 °C without water, noise, or messy cables using a unique and fully integrated air-cooled Peltier system.
Control UV-Vis thermal ramp measurements at up to 40 °C/min using highly accurate, solid-state digital Cary temperature probes that control experimental temperature from inside the cuvette.
Ensure analytical accuracy for small-volume measurements with a highly-collimated and uniform beam of less than 1.5 mm width, coupled with permanent optical alignment of the stationary cell holders.
Reduce data processing time and calculation errors with more than 50 built-in and customized calculations that can be saved and applied automatically to any method used routinely.
Achieve research grade photometric performance, stability, and range with a double beam spectrophotometer featuring leading optical technologies.
Be up and running in minutes by following the intuitive video tutorials in Cary UV Workstation’s Help and Learning Center.
Meet data integrity standards—provides the technical controls to securely acquire, process, report and store data in laboratories that must follow the compliance guidelines of FDA 21 CFR Part 11, EU Annex 11, GAMP5, as well as ISO/IEC 17025 and EPA’s 40 CFR Part 160.
Store and manage your Cary 3500 Multicell UV-Vis spectrophotometer data in a local secured storage on the workstation PC, or in a central storage location.
Deploy the OpenLab software suite and get optional secure database storage for electronic records, complete administration for users and access privileges, software licensing, and security settings—e-signature workflow and enhanced audit trail review capabilities.
Choose sustainability: The Cary 3500 Multicell UV-Vis received My Green Lab’s ACT (Accountability, Consistency, Transparency) label after independent audit for its environmental impact throughout the product lifecycle.
Specifications
| Compliance Software | OpenLab Software Suite |
| Control Software | Cary UV Workstation Cary 3500 Multizone Add-on |
| Depth | 700mm |
| Height | 280mm |
| Light Source | Xenon flash lamp (250 Hz) with 10-year warranty on new instruments |
| Maximum Scanning Speed | UV-Vis 150,000 nm/min |
| Photometric Range (Abs) | 4Abs |
| Photometric System | Double beam |
Double beam | 100-240 VAC, 50/60 Hz |
| Spectral Bandwidth | UV-Vis 0.1 - 5.0 nm |
| Wavelength | 190 - 1100 nm |
| Weight | 34kg |
| Weight | 445mm |
| Z-height | 15mm |
Applications
Quantifying a molecule—concentration measurements
QC processes in the pharmaceutical industry often use this reliable, well-established approach, and data integrity is crucial. The Agilent Cary 3500 Multicell UV-Vis spectrophotometer simultaneously measures samples, standards, and controls, eliminating most analytical variables.
Read the application note: Improve Data Integrity and Remove Analytical Variables in UV-Vis Measurements.
Conformational studies of proteins and DNA/RNA
Structural or conformational transitions are often associated with changes in UV-Vis absorbance. UV-Vis spectroscopy can study conformational changes in molecules such as proteins and DNA/RNA. UV-Vis is often used in protein and nucleic acid thermal melt analyses, and sample temperature control is key in ID testing. Read the application note and case study to see how the Cary 3500 UV-Vis allows accurate, reproducible ramping up to 40 °C/min. Or browse our comprehensive guide to thermal melt analysis by UV-Vis.
Monitoring reaction kinetics with UV-Vis
UV-Vis is ideal for analyzing reaction kinetics when the change in reactant or products produces a change in absorbance at a specific wavelength over time. Single-point kinetics monitors a single wavelength, and scanning kinetics monitors a wavelength range. The Cary 3500 Multicell UV-Vis collects data quickly (up to 250 data points per second) —perfect for scanning kinetics measurements. Watch the video to see how the Cary 3500 measures multiple samples at different temperatures simultaneously.
